lc-ms/ms法檢測皮質類固醇ace 3 c18-pfp: corticosteroids by lc-ms/ms
ace c18-pfp, 3μm, 150 x 2.1mm
gradient analysis
a: 0.1% formic acid in water
b: ch3cn – 0.1% formic acid in water (90:10)
time (mins) %b
0 30
14 50
17 95
20 30
flow rate: 0.3ml/min
column temperature: 15°c
injection volume: 25μl
turbospray, mrm
1. triamcinolone
2. prednisolone
3. fluoroprednisolone
4. methylprednisolone
5. betamethasone
6. dexamethasone
7. flumethasone