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二手安捷倫hplc液相色譜儀分析中保留時(shí)間不重現(xiàn)是因?yàn)槭裁矗慷职步輦恏plc液相色譜儀是分析實(shí)驗(yàn)室常用的測(cè)試儀器之一，其應(yīng)用越來(lái)越廣泛。此種儀器在使用過(guò)程中，難免會(huì)出現(xiàn)各種各樣的問(wèn)題，并將直接影響到所測(cè)數(shù)據(jù)的準(zhǔn)確性和儀器的正常工作。操作者如果能了解故障的成因，即可清楚預(yù)防和排除這些故障的方法，就可正確地使用儀器并大限度地發(fā)揮儀器的性能。今天我們來(lái)說(shuō)下液相色譜儀分析中保留時(shí)間不重現(xiàn)的可能原因。<br>1、確認(rèn)流動(dòng)相是否新鮮配制的，比例有無(wú)錯(cuò)誤。<br>2、確認(rèn)色譜條件是否在色譜柱使用范圍內(nèi)，如ph范圍、溫度和壓力。<br>3、仔細(xì)觀察柱前壓的變化。如果壓力穩(wěn)定，可以判斷泵系統(tǒng)沒(méi)有問(wèn)題。如果壓力不穩(wěn)定，二手安捷倫hplc液相色譜儀通常是由于泵內(nèi)有氣泡造成的，一定會(huì)影響保留時(shí)間。將流動(dòng)相仔細(xì)脫氣，并排出泵內(nèi)氣泡。<br>4、觀察色譜峰形和柱效變化情況，確認(rèn)色譜柱是否被污染。如果色譜柱被污染，可使用適當(dāng)溶劑沖洗。<br>5、如果同時(shí)伴有色譜柱柱壓升高，降低柱壓。<br>6、如果使用梯度洗脫，確認(rèn)平衡時(shí)間是否足夠長(zhǎng)。流動(dòng)相中使用離子對(duì)試劑（烷基磺酸鈉等）或固定相為正相硅膠等往往需要更長(zhǎng)的平衡時(shí)間。<br>7、確認(rèn)樣品是否過(guò)載。<br>8、二手安捷倫hplc液相色譜儀組分在色譜柱中保留不夠。調(diào)整色譜條件，使樣品在柱上適當(dāng)保留。<br> <div class="zbbbnpu2h4x" id="contentb"></div> </div> <div id="zbbbnpu2h4x" class="clearfix"></div> </div> <div>上一個(gè)：<a 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