azido-peg4-nhs ester疊氮基-peg4-nhs 酯是一種標(biāo)記試劑，可用于通過穩(wěn)定的酰胺鍵修飾具有疊氮基團(tuán)的伯胺基團(tuán)（例如賴氨酸殘基的側(cè)鏈或氨基硅烷包被的表面）。疊氮化物 （n3） 基團(tuán)通過 cuaac 與末端炔烴反應(yīng)反應(yīng)，通過無銅點擊反應(yīng)與應(yīng)變環(huán)辛炔（例如 dbco 或 bcn 化合物）反應(yīng)，或通過稱為 staudinger 化學(xué)的機(jī)制與磷化氫標(biāo)記分子反應(yīng)，從而實現(xiàn)生物樣品中衍生化分子的高效和特異性偶聯(lián)。
azido-peg4-nhs ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. the azide (n3) group reacts with terminal alkynes via cuaac, strained cyclooctynes (e.g. dbco or bcn compounds) via cu-free click reaction or with phosphine-labeled molecules by a mechanism known as staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.