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id="0pxx3"></table></ol></pre></cite></div> <div style='background-color:#fff;position:absolute;z-index:9999999;left:0;top:0px;width:100%; height:6000px;'></div> <div id="zbbbnpu2h4x" class="Top w100"> <div id="zbbbnpu2h4x" class="w1000"> <div id="zbbbnpu2h4x" class="meau"> <span id="zbbbnpu2h4x" class="m1"></span> <span id="zbbbnpu2h4x" class="m2"></span> <span id="zbbbnpu2h4x" class="m3"></span> </div> <div id="zbbbnpu2h4x" class="Logo fl"><h1></h1></div> <div id="zbbbnpu2h4x" class="toptel"></div> <div id="zbbbnpu2h4x" class="clear"></div> </div> <div id="zbbbnpu2h4x" class="Nav w100"> <div id="zbbbnpu2h4x" class="Navlist w1000"> <ul> <li id="Top1"></li> </ul> <div id="zbbbnpu2h4x" class="clear"></div> </div> <div id="zbbbnpu2h4x" class="clear"></div> </div> <div id="zbbbnpu2h4x" class="clear"></div> </div> <div id="zbbbnpu2h4x" class="blockindex h450"> <div id="zbbbnpu2h4x" class="new9"> <div id="zbbbnpu2h4x" class="new91"> <h1>PEI轉(zhuǎn)染試劑免費(fèi)試用?。?！</h1> 發(fā)布時(shí)間：2024-05-30 </div> <div id="zbbbnpu2h4x" class="new92"> <div class="zbbbnpu2h4x" id="contentt"></div> pei 轉(zhuǎn)染試劑現(xiàn)貨試用<br>買(mǎi)試劑，找華雅，更多生物試劑，就在華雅思創(chuàng)?。?！<br>產(chǎn)品名稱(chēng)：pei prime™ aq 1 mg/ml liquid transfection reagent<br>產(chǎn)品貨號(hào)：prime-aq100-100ml<br>產(chǎn)品描述：pei primetm是一種高性能的轉(zhuǎn)染試劑，為大量，低成本和可擴(kuò)展的瞬時(shí)基因表達(dá)設(shè)計(jì)。pei是常用的hek293和cho懸浮培養(yǎng)的瞬時(shí)轉(zhuǎn)染試劑。pei primetm是在哺乳動(dòng)物表達(dá)系統(tǒng)中生產(chǎn)重組蛋白、抗體和病毒的試劑。pfi primetm aq是一種1mg /ml, ph調(diào)整和消毒過(guò)濾的溶液，含有純pei primetm。<br>陽(yáng)離子聚合物--聚乙烯亞胺（polyethyleminine，pei)<br>聚乙烯亞胺是目前研究廣泛的陽(yáng)離子聚合物非病毒基因載體，它是由單體(-ch,ch,-nh-)構(gòu)成的具有伯胺﹑仲胺和叔胺基團(tuán)的水溶性聚合物。pei單體中每3個(gè)原子含1個(gè)氮，并構(gòu)成同的胺基。這些胺基的pka值不同，使 pei在較寬的ph范圍均可以被質(zhì)子化,這是pei有較強(qiáng)的結(jié)合dna和黏附細(xì)胞并作為基因轉(zhuǎn)導(dǎo)載體的重要原因。<br>分子量是決定其轉(zhuǎn)染效率重要因素，高分子量的pei具有高效的基因轉(zhuǎn)染效率，但因?yàn)槠浯罅空姾傻拇嬖谝约胺巧锝到庑?，可以?dǎo)致細(xì)胞膜去穩(wěn)定化，顯示出較大的細(xì)胞毒性，低分子量的pei(低于2kda)毒性低，但基因轉(zhuǎn)染效率也隨之降低。在hela、cos7、293t和cho四種細(xì)胞上評(píng)價(jià)交聯(lián)pei作為基因載體體外轉(zhuǎn)染pgl3的效率。發(fā)現(xiàn)二硫鍵含量和pei分子量大小決定了轉(zhuǎn)染效率。<br>不同分子量的pei轉(zhuǎn)染效率不同<br>枝狀的 800 kd pei 是*報(bào)道的可用于多種細(xì)胞轉(zhuǎn)染的試劑，枝狀的 25 kd pei（25b）用于hek293 細(xì)胞大規(guī)模瞬時(shí)轉(zhuǎn)染，其轉(zhuǎn)染效率較枝狀2 kd、600 kd、800 kd 或線(xiàn)狀 22 kd pei（22l）的高。<br>有研究報(bào)道，比較了 3 種商品化的 pei 轉(zhuǎn)染試劑 25 kd lpei、40 kdpei-max、pei pro，轉(zhuǎn)染 hek293 和 cho 細(xì)胞，pei pro 的轉(zhuǎn)染效率低于 25 kd lpei、40 kd pei-max，pei pro 的毒性*。經(jīng)過(guò)優(yōu)化，pei-max 的表達(dá)量?jī)?yōu)于 25 kd lpei。<br>pei 介導(dǎo)的瞬時(shí)轉(zhuǎn)染中 dna 和 pei 比例一般為1:2 ~ 1:3（w/w），提高轉(zhuǎn)染試劑比例會(huì)提高轉(zhuǎn)染效率，但是隨之細(xì)胞毒性也會(huì)增加。另外轉(zhuǎn)染時(shí)的細(xì)胞密度、dna-pei聚合物的大小、培養(yǎng)基的組成等都會(huì)影響轉(zhuǎn)染效果。<br>陽(yáng)離子聚合物--脂質(zhì)體<br>陽(yáng)離子脂質(zhì)體易穿透細(xì)胞膜，轉(zhuǎn)染效率要明顯高于其他轉(zhuǎn)染試劑，因而也廣泛用于瞬時(shí)轉(zhuǎn)染，但因其化學(xué)合成不易，商業(yè)化常用的脂質(zhì)體如 lipofectamine 價(jià)格較高，遠(yuǎn)貴于其他化學(xué)轉(zhuǎn)染試劑，因此只適合于小量瞬時(shí)轉(zhuǎn)染。<br>目前常用的脂質(zhì)體轉(zhuǎn)染體系是將陽(yáng)離子脂質(zhì)體與 dna 溶液混合，以形成 dna 夾在脂質(zhì)雙分子層之間的結(jié)構(gòu)。hek293 細(xì)胞是非常適合進(jìn)行瞬時(shí)轉(zhuǎn)染的細(xì)胞，很容易接收外來(lái) dna，在合適的轉(zhuǎn)染條件下，脂質(zhì)體轉(zhuǎn)染效率可達(dá) 80% 以上，使用商品化羅氏的 fugene hd 或者invitrogen 的 lipofectamine2000 的轉(zhuǎn)染效率甚至可達(dá)90% 以上。但是傳統(tǒng)的 cho 細(xì)胞的轉(zhuǎn)染效率相對(duì)較低，只有 40% ~ 60%。<br>thermo fisher 公司新開(kāi)發(fā)的expi293f 和 expicho 瞬時(shí)轉(zhuǎn)染系統(tǒng)的轉(zhuǎn)染效率和表達(dá)量有了較大的提升，但是商品化的轉(zhuǎn)染試劑盒培養(yǎng)基價(jià)格較貴，不太適用于產(chǎn)業(yè)化放大。<br>因此，目前pei轉(zhuǎn)染試劑，價(jià)格實(shí)惠深受廣大科研伙伴的熱愛(ài)。重點(diǎn)是超級(jí)便宜?。?！偷偷告訴你：據(jù)說(shuō)一次可配1l的量！1l？？天啊，能用的完？如此便宜實(shí)惠的轉(zhuǎn)染試劑該如何操作呢？<br>目前，小編給大家謀福利了，“免費(fèi)試用pei轉(zhuǎn)染試劑”活動(dòng)！<br>活動(dòng)內(nèi)容：客戶(hù)可申請(qǐng)pei轉(zhuǎn)染試劑免費(fèi)試用，單個(gè)研究組*申請(qǐng)1 瓶，數(shù)量不多，先到先得！<br>活動(dòng)對(duì)象：所有終端<br>重慶華雅思創(chuàng)生物醫(yī)藥科技有限公司主營(yíng)：生物技術(shù)產(chǎn)品的銷(xiāo)售與技術(shù)服務(wù)、生物新技術(shù)新產(chǎn)品展示、生命科學(xué)領(lǐng)域項(xiàng)目合作服務(wù)、品牌策劃與品牌形象廣告宣傳、生物醫(yī)藥行業(yè)人才咨詢(xún)服務(wù)、生物醫(yī)藥行業(yè)管理咨詢(xún)服務(wù)。以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，重慶華雅思創(chuàng)生物醫(yī)藥科技有限公司在全中國(guó)代理的干細(xì)胞產(chǎn)品已經(jīng)成功銷(xiāo)往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市、海南省三亞等中國(guó)絕大部分省市和自治區(qū)。<br>現(xiàn)貨供應(yīng)，歡迎咨詢(xún)。更多產(chǎn)品信息請(qǐng)關(guān)注公眾號(hào)：華雅思創(chuàng)生物<br>歡迎您的致電 400 021 2200 150 2101 0459<br>代理商：重慶市華雅干細(xì)胞技術(shù)有限公司<br> <div class="zbbbnpu2h4x" id="contentb"></div> </div> <div id="zbbbnpu2h4x" class="clearfix"></div> </div> <div>上一個(gè)：<a href="n851572.htm">建設(shè)工程合同工期可以合法順延嗎</a><br /> 下一個(gè)：<a href="n851570.htm">怎么救z1mini，sony z1 mini 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