巧克力分析
chocolate analysis
conditions
column: ace excel 2 c18-amide
dimensions: 100 x 2.1 mm
part number: exl-1012-1002u
mobile phase:
a: 10 mm ammonium formate ph 2.8 in h2o
b: 10 mm ammonium formate ph 2.8 in mecn/h2o (90:10 v/v)
time(mins)
%b
0.0
5
1.5
85
flow rate: 1.2 ml/min
temperature: 42 °c
detection: uv, 254 nm