維生素-水溶性（梯度ii）分析conditions
column: ace 5 c18
dimensions: 250 x 4.6 mm
part number: ace-121-2546
mobile phase:
a: 50 mm kh2po4 ph 3.0 in h2o
b: meoh
gradient:
time(mins)
%b
0
3
5
3
45
45
50
80
flow rate: 1 ml/min
temperature: ambient
detection: uv, 205 nm
analytes
1. pyridoxamine
2. thiamine (vitamin b1)
3. l -ascorbic acid (vitamin c)
4. nicotinic acid
5. pyridoxal
6. impurity
7. pyridoxine
8. p-aminobenzoic acid
9. pantothenic acid (vitamin b5)
10. folic acid (vitamin b9)
11. cyanocobalamin (vitamin b12)
12. d-biotin (vitamin b7)
13. riboflavin (vitamin b2)