nmt是基因功能的活體檢測(cè)技術(shù)，已被31位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。
期刊：chemosphere
主題：浮萍超富集cd的分子機(jī)制研究
標(biāo)題：comparative transcriptome analysis of duckweed (landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation
影響因子：4.427
檢測(cè)指標(biāo)：cd2+流速
檢測(cè)樣品：浮萍根（根尖、距離根尖400μm，1000μm）
cd2+流速流實(shí)驗(yàn)處理方法：
50 μm cdcl2處理30min
cd2+流速流實(shí)驗(yàn)測(cè)試液成份：
0.1 mm kcl，0.1 mm mgcl2、0.05 mm cacl2、0.3 mm mes、50μm cdcl2，ph5.5
作者：中科院青島生物能源與過(guò)程研究所周功克、徐華
英文摘要
cadmium (cd) is a detrimental environmental pollutant. duckweeds have been considered promising candidates for cd phytoremediation. although many physiological studies have been conducted, the molecular mechanisms underlying cd hyperaccumulation in duckweeds are largely unknown.
in this study, clone 6001 of landoltia punctata, which showed high cd tolerance, was obtained by large-scale screening of over 200 duckweed clones. subsequently, its growth, cd flux, cd accumulation, and cd distribution characteristics were investigated. to further explore the global molecular mechanism, a comprehensive transcriptome analysis was performed. for rna-seq, samples were treated with 20 μm cdcl2 for 0, 1, 3, and 6 days.
in total, 9,461, 9,847, and 9615 differentially expressed unigenes (degs) were discovered between cd-treated and control (0 day) samples. deg clustering and enrichment analysis identified several biological processes for coping with cd stress. genes involved in dna repair acted as an early response to cd, while rna and protein metabolism would be likely to respond as well.
furthermore, the carbohydrate metabolic flux tended to be modulated in response to cd stress, and upregulated genes involved in sulfur and ros metabolism might cause high cd tolerance. vacuolar sequestration most likely played an important role in cd detoxification in l. punctata 6001. these novel findings provided important clues for molecular assisted screening and breeding of cd hyperaccumulating c*rs for phytoremediation.
中文摘要（谷歌機(jī)翻）
鎘（cd）是有害的環(huán)境污染物。浮萍被認(rèn)為是鎘植物修復(fù)的有前途的候選人。盡管已進(jìn)行了許多生理研究，但浮萍中cd過(guò)度富集的分子機(jī)制尚不清楚。
在這項(xiàng)研究中，通過(guò)大規(guī)模篩選200多個(gè)浮萍克隆，獲得了對(duì)鎘具有高耐受性的landoltia punctata克隆6001。隨后，研究了其生長(zhǎng)，cd通量，cd積累和cd分布特征。為了進(jìn)一步探索全局分子機(jī)制，進(jìn)行了全面的轉(zhuǎn)錄組分析。對(duì)于rna-seq，樣品用20μmcdcl2處理0、1、3和6天。
在經(jīng)鎘處理的樣品和對(duì)照（0天）樣品之間總共發(fā)現(xiàn)了9,461、9,847和9615個(gè)差異表達(dá)的單基因（deg）。 deg聚類和富集分析確定了應(yīng)對(duì)cd脅迫的幾種生物學(xué)過(guò)程。參與dna修復(fù)的基因是對(duì)cd的早期反應(yīng)，而rna和蛋白質(zhì)代謝也可能也有響應(yīng)。
此外，碳水化合物的代謝通量?jī)A向于響應(yīng)cd脅迫而被調(diào)節(jié)，而參與硫和ros代謝的基因上調(diào)可能導(dǎo)致對(duì)cd的高耐受性。液泡螯合有可能在點(diǎn)球藻6001的cd解毒中發(fā)揮重要作用。這些新發(fā)現(xiàn)為分子輔助篩選和育種cd超富集植物進(jìn)行植物修復(fù)提供了重要線索。
結(jié)果表明：葉片（f）的cd2+吸收速率高于根冠（r0和r1），低于根伸長(zhǎng)區(qū)（r2）。葉片中，從節(jié)點(diǎn)（f2）到頂點(diǎn)（f0）的cd2+吸收速率逐漸下降。根和葉片的cd2+吸收速率顯著較低,從而更容易適應(yīng)cd脅迫。